7QXR

Azacoelenterazine-bound Renilla-type luciferase (AncFT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	51.284, 87.549, 102.693
Unit cell angles	90.00, 93.46, 90.00

Refinement procedure

Resolution	46.944 - 2.052
R-factor	0.1952
Rwork	0.192
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s97
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.944	2.110
High resolution limit [Å]	2.050	2.050
Number of reflections	56440	4304
<I/σ(I)>	9.8
Completeness [%]	99.5
Redundancy	6.9
CC(1/2)	0.997	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	magnesium chloride, Bis-tris, PEG 3350