7QXM

Crystal structure of the Vibrio cholerae replicative helicase (DnaB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	141.230, 150.099, 188.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.630 - 3.800
R-factor	0.2659
Rwork	0.266
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6t66
RMSD bond length	0.004
RMSD bond angle	0.610
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XSCALE (Mar 15, 2019)
Phasing software	MOLREP (Vers 11.7.01)
Refinement software	BUSTER (2.10.3 (20-MAY-2020))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.630	32.630	3.900
High resolution limit [Å]	3.800	16.990	3.800
Rmerge	0.408	0.077	3.583
Rmeas	0.424	0.081	3.719
Total number of observations	551596
Number of reflections	40084	496	2925
<I/σ(I)>	4.84	24.53	0.27
Completeness [%]	99.9	94.8	100
Redundancy	13.761	11.581	13.967
CC(1/2)	0.995	0.998	0.241

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	0.1M Sodium Acetate 1.2M Sodium Malonate