7QW4
Pden_5119 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 134.510, 136.376, 212.314 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.750 - 3.100 |
R-factor | 0.3202 |
Rwork | 0.320 |
R-free | 0.32120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model |
RMSD bond length | 0.014 |
RMSD bond angle | 2.094 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 3.140 |
High resolution limit [Å] | 3.060 | 3.060 |
Rmerge | 0.310 | |
Rmeas | 0.438 | |
Rpim | 0.060 | |
Number of reflections | 65429 | 3022 |
<I/σ(I)> | 9.7 | 2.1 |
Completeness [%] | 90.2 | 57.6 |
Redundancy | 2 | |
CC(1/2) | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 303.15 | 50 mM trisodium phosphate, 300 mM sodium chloride, 300 mM imidazole, pH 8.0 |