Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96872 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.767, 105.767, 185.882 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.324 - 3.100 |
| R-factor | 0.2747 |
| Rwork | 0.275 |
| R-free | 0.32990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5my6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.406 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.324 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 22490 | 4001 |
| <I/σ(I)> | 5.1 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.2 | 19.7 |
| CC(1/2) | 0.990 | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 13% PEG6000 (w/v), 0.1 M MES pH 6.5, 7% 2-methyl-2,4-pentanediol |
