7QVD
X-ray structure of the lytic transglycosylase SltB2 from Pseudomonas aeruginosa
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.966, 42.395, 93.579 |
| Unit cell angles | 90.00, 95.65, 90.00 |
Refinement procedure
| Resolution | 46.560 - 1.700 |
| Rwork | 0.164 |
| R-free | 0.19530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5anz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.560 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 | 1.072 |
| Rpim | 0.036 | 0.435 |
| Number of reflections | 36814 | 1926 |
| <I/σ(I)> | 13 | 2.3 |
| Completeness [%] | 99.0 | 99.3 |
| Redundancy | 6.6 | 6.9 |
| CC(1/2) | 0.998 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% Polyethylene Glycol 4000, 0.1 M sodium acetate and 8% 2-propanol |
