7QUA
Duplex RNA containing Xanthosine-Cytosine base pairs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 41.070, 35.150, 31.930 |
| Unit cell angles | 90.00, 129.05, 90.00 |
Refinement procedure
| Resolution | 24.800 - 1.000 |
| R-factor | 0.1384 |
| Rwork | 0.137 |
| R-free | 0.15760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q1r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.980 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Number of reflections | 220228 | 470 |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 90.0 | |
| Redundancy | 11.2 | |
| CC(1/2) | 1.000 | 0.050 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Na cacodylate pH7.0, MPD, spermine, NaCl, MgCl2 |
