7QS8
Structural insight into the Scribble PDZ domains interaction with the oncogenic Human T-cell lymphotrophic virus-1 (HTLV-1) Tax1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.389, 43.795, 60.073 |
| Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
| Resolution | 31.140 - 1.850 |
| Rwork | 0.226 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vwi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.196 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER (1.15.2-3472-000) |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.220 | 1.910 |
| High resolution limit [Å] | 1.850 | 1.870 |
| Rmerge | 0.064 | 1.320 |
| Number of reflections | 16499 | 1313 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M Hepes ph 7, 30% v/v Jeffamine |
