7QS3

Crystal structure of B30.2 PRYSPRY domain of TRIM16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00000
Spacegroup name	P 43 21 2
Unit cell lengths	55.730, 55.730, 159.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.690 - 1.750
R-factor	0.1647
Rwork	0.163
R-free	0.19790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6flm
RMSD bond length	0.015
RMSD bond angle	1.448
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.690	45.690	1.810
High resolution limit [Å]	1.750	6.780	1.750
Rmerge	0.058	0.042	0.992
Rmeas	0.065	0.045	1.123
Rpim	0.021	0.016	0.366
Number of reflections	26339	565	2509
<I/σ(I)>	16.3		2.3
Completeness [%]	99.9	99.8	99.9
Redundancy	9	7.7	9.1
CC(1/2)	0.999	0.999	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	20% PEG3350, 10% ethylene glycol, 0.1M bis-tris-propane pH 6.5, 0.2M sodium bromide