7QRV

Crystal structure of NHL domain of TRIM2 (full C-terminal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00004
Spacegroup name	P 1 21 1
Unit cell lengths	68.669, 89.242, 86.725
Unit cell angles	90.00, 106.99, 90.00

Refinement procedure

Resolution	45.470 - 1.450
R-factor	0.1531
Rwork	0.152
R-free	0.17790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b2r
RMSD bond length	0.016
RMSD bond angle	1.636
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.470	45.430	1.490
High resolution limit [Å]	1.450	6.480	1.450
Rmerge	0.059	0.043	0.411
Rmeas	0.069	0.046	0.488
Rpim	0.026	0.017	0.186
Number of reflections	175817	2065	12684
<I/σ(I)>	16.8		3.8
Completeness [%]	99.5	99.7	97.4
Redundancy	6.8	6.9	6.7
CC(1/2)	0.999	0.998	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	30% w/v PEG 5000 MME, 0.2 M Ammonium sulfate, 0.1M MES, pH 6.5