7QR1
SpCas9 bound to TRAC off-target2 DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-20 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.420, 66.795, 187.240 |
| Unit cell angles | 90.00, 111.41, 90.00 |
Refinement procedure
| Resolution | 47.540 - 2.600 |
| R-factor | 0.2072 |
| Rwork | 0.206 |
| R-free | 0.23070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fq5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.577 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.140 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.200 | 2.300 |
| Number of reflections | 63694 | 6348 |
| <I/σ(I)> | 9.4 | 0.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.8 | 7.1 |
| CC(1/2) | 0.997 | 0.318 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350 |
