7QQM

The PDZ domain of LRRC7 fused with ANXA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	59.780, 70.080, 102.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.481 - 1.600
R-factor	0.1711
Rwork	0.170
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.481	1.640
High resolution limit [Å]	1.600	1.600
Rmeas	0.106	1.952
Number of reflections	57570	4207
<I/σ(I)>	18.28	1.57
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	1.000	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Potassium thiocyanate 0.1 M Sodium bromide 0.1 M MES 6.5 12 % v/v PEG Smear High