7QQL

The PDZ domain of SNTG2 complexed with the phosphorylated PDZ-binding motif of RSK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	97.460, 60.550, 135.210
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	48.416 - 2.440
R-factor	0.197
Rwork	0.195
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.416	2.500
High resolution limit [Å]	2.440	2.440
Rmeas	0.147	1.655
Number of reflections	59163	4350
<I/σ(I)>	11.3	1.18
Completeness [%]	99.9
Redundancy	6.79
CC(1/2)	0.998	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Succinic acid pH 7.0 15% w/v PEG 3350