7QQF

Crystal structure of unliganded MYORG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97951
Spacegroup name	P 1
Unit cell lengths	74.109, 78.986, 180.113
Unit cell angles	88.06, 78.80, 62.71

Refinement procedure

Resolution	66.570 - 2.430
Rwork	0.211
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f2h
RMSD bond length	0.005
RMSD bond angle	1.377
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.570	66.570	2.470
High resolution limit [Å]	2.430	13.310	2.430
Rmerge	0.066	0.015	1.071
Rmeas	0.093	0.021	1.514
Rpim	0.066	0.015	1.071
Number of reflections	131515	796	6509
<I/σ(I)>	8.2	29.8	1
Completeness [%]	98.2
Redundancy	2.2	2.1	2.1
CC(1/2)	0.983	0.964	0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	100 mM HEPES pH 7.0, 10% PEG MME5000 and 5% tasimate pH 7.0.