7QQC
Structure of CTX-M-15 K73A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.72 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.896, 45.552, 116.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.910 - 0.950 |
| R-factor | 0.1405 |
| Rwork | 0.140 |
| R-free | 0.15550 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6qw8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.430 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rpim | 0.043 | 0.794 |
| Number of reflections | 151252 | 7462 |
| <I/σ(I)> | 8.2 | 0.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13 | |
| CC(1/2) | 0.999 | 0.368 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | 2.0 M ammonium sulphate, 0.1 M Tris 8.0 |
