7QPX

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikC with an engineered HMA domain of Pikp-1 (Pikp-SNK-EKE) from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	66.353, 83.130, 107.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.710 - 2.050
R-factor	0.2056
Rwork	0.204
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a8w
RMSD bond length	0.008
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	Aimless (0.6.1)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.710	46.710	2.110
High resolution limit [Å]	2.050	8.940	2.050
Rmerge	0.081	0.038	1.048
Rmeas	0.085	0.041	1.103
Rpim	0.027	0.014	0.340
Number of reflections	37795	534	2886
<I/σ(I)>	15.2
Completeness [%]	99.8	99.2	99.4
Redundancy	9.9	8	10.1
CC(1/2)	0.999	0.998	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NULL