7QOX

Factor XI and Plasma Kallikrein apple domain structures reveals different kininogen bound complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	93
Detector technology	PIXEL
Collection date	2015-05-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	55.543, 68.258, 120.311
Unit cell angles	90.00, 96.55, 90.00

Refinement procedure

Resolution	29.640 - 2.320
R-factor	0.2019
Rwork	0.199
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i58
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.640	2.400
High resolution limit [Å]	2.320	2.320
Number of reflections	38760	9002
<I/σ(I)>	7.6
Completeness [%]	99.4
Redundancy	3.4
CC(1/2)	0.987	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		288	Morpheus screen H9 condition: 0.1M amino acids, 0.1M buffer system 3 pH8.5, 30% precipitant mix 1.