7QO8

Structure of Protease1 from Pyrococcus horikoshii in space group 19 with a hexamer in the asymmetric unit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9797
Spacegroup name	P 21 21 21
Unit cell lengths	115.741, 123.636, 129.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.590 - 1.950
R-factor	0.1818
Rwork	0.181
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q3t
RMSD bond length	0.009
RMSD bond angle	1.103
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.720	2.050
High resolution limit [Å]	1.950	1.950
Rpim	0.068	0.673
Number of reflections	135285	19324
<I/σ(I)>	11.8
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.997	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM tri-sodium citrate pH 5.6, 200 mM sodium potassium tartrate, 1.9 to 2.4 M Ammonium sulfate