7QNX

The receptor binding domain of SARS-CoV-2 spike glycoprotein in complex with Beta-55 and EY6A Fabs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 32 2 1
Unit cell lengths	131.772, 131.772, 116.837
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	81.640 - 2.920
R-factor	0.2304
Rwork	0.229
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nx8
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.000	2.970
High resolution limit [Å]	2.920	2.920
Rmerge	0.554
Rpim	0.125	0.902
Number of reflections	25801	1228
<I/σ(I)>	3.8	0.5
Completeness [%]	99.9
Redundancy	20.5
CC(1/2)	0.986	0.311

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NaCl, 0.1 M Tris, pH 6.7 and 30% (w/v) PEG 3000