7QNQ

Structure of the Aux2 relaxosome protein of plasmid pLS20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	137.854, 137.854, 121.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.650 - 1.890
R-factor	0.1814
Rwork	0.180
R-free	0.20100
Structure solution method	AB INITIO PHASING
RMSD bond length	0.005
RMSD bond angle	0.662
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless (0.7.4)
Phasing software	Arcimboldo
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.480	1.960
High resolution limit [Å]	1.890	1.890
Rmerge	0.190	3.150
Rmeas	0.200	3.100
Number of reflections	87560	4373
<I/σ(I)>	14.3	1.5
Completeness [%]	96.1	57.4
Redundancy	26.5	26.5
CC(1/2)	0.998	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		300	0.06 M D-Glucose, 0.06 M D-Mannose, 0.06 M D-Galactose, 0.06 M L-Fucose, 0.06 M D-Xylose, 0.06 M N-Acetyl-D-Glucosamine, 0.06 M Tris-Bicine pH8.5, 20% v/v Ethylene glycol, 10 % w/v PEG 8000