7QNP
Designed Armadillo repeat protein N(A4)M4C(AII) co-crystallized with hen egg white lysozyme
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.592, 62.655, 108.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.997 - 1.586 |
| Rwork | 0.186 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aei |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.650 |
| High resolution limit [Å] | 1.586 | 1.586 |
| Rmerge | 0.056 | |
| Rmeas | 0.059 | |
| Rpim | 0.016 | 0.384 |
| Number of reflections | 53083 | 5191 |
| <I/σ(I)> | 22.36 | 1.48 |
| Completeness [%] | 100.0 | 99.98 |
| Redundancy | 13.4 | 13.8 |
| CC(1/2) | 1.000 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 35% (v/v) dioxane, pH undefined |
