7QNO
Crystal structure of ligand-free Danio rerio HDAC6 CD1 CD2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-30 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 186.134, 186.134, 102.442 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 63.340 - 2.380 |
R-factor | 0.2132 |
Rwork | 0.212 |
R-free | 0.22780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g0h |
RMSD bond length | 0.004 |
RMSD bond angle | 0.720 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.067 | 2.683 |
High resolution limit [Å] | 2.377 | 2.377 |
Rmerge | 0.153 | 2.331 |
Rpim | 0.035 | 0.533 |
Number of reflections | 44105 | 2206 |
<I/σ(I)> | 15.7 | |
Completeness [%] | 95.2 | 73.3 |
Redundancy | 20.4 | 19.6 |
CC(1/2) | 0.999 | 0.488 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.27 M sodium formate |