7QJI
X-Ray Structure of apo-EleNRMT in complex with two Nanobodies at 4.1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-01-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999869 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.270, 122.250, 149.110 |
Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
Resolution | 11.990 - 4.100 |
R-factor | 0.2693 |
Rwork | 0.267 |
R-free | 0.31550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 7QIA |
RMSD bond length | 0.003 |
RMSD bond angle | 0.603 |
Data reduction software | XDS (Version Feb5 2021) |
Data scaling software | XSCALE (Version Feb5 2021) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 4.200 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmeas | 0.112 | 1.536 |
Number of reflections | 25065 | 1742 |
<I/σ(I)> | 15.36 | 1.81 |
Completeness [%] | 99.2 | 98.9 |
Redundancy | 28.2 | 27.1 |
CC(1/2) | 0.999 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 50 mM MgAc, 50 mM HEPES pH 7.2-7.6 and 25-30% PEG400 |