7QFO
Human Topoisomerase II Beta ATPase AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.021, 84.021, 127.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.412 - 1.900 |
| Rwork | 0.193 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zxm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.729 |
| Data reduction software | DIALS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.420 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 36646 | 2300 |
| <I/σ(I)> | 23.3 | 1.2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 48 | 50.9 |
| CC(1/2) | 1.000 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M PCTP (pH 7.0), 25 % w/v PEG 1500 |
