7QFN
Human Topoisomerase II Beta ATPase ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 103.021, 103.021, 66.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 89.219 - 2.621 |
| Rwork | 0.199 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1292119290 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.935 |
| Data reduction software | DIALS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.220 | 2.740 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Number of reflections | 12596 | 1513 |
| <I/σ(I)> | 9 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 11 |
| CC(1/2) | 0.997 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium sulphate, 0.1 M bis tris (pH 5.5), 25 % w/v PEG 3350 |
