7QF2
Structure of miniSOG reconstituted with riboflavin as a cofactor
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.973 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 39.900, 39.900, 134.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.243 - 1.070 |
Rwork | 0.130 |
R-free | 0.15530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gpu |
RMSD bond length | 0.019 |
RMSD bond angle | 2.200 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.250 | 1.100 |
High resolution limit [Å] | 1.070 | 1.070 |
Rmeas | 0.090 | 1.120 |
Number of reflections | 91259 | 6739 |
<I/σ(I)> | 11.5 | 1.12 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 6.5 | 5.6 |
CC(1/2) | 0.990 | 0.563 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM Tris-HCl pH 8.0, 20 mM MgCl2, 28% PEG 4000, 15 mM CoCl2 |