7QDP
Crystal structure of FLT3 T343I in complex with the canonical ligand FL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 102.428, 113.377, 123.225 |
| Unit cell angles | 105.37, 109.47, 108.22 |
Refinement procedure
| Resolution | 48.580 - 3.691 |
| R-factor | 0.258 |
| Rwork | 0.257 |
| R-free | 0.27650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qs7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.580 | 3.822 |
| High resolution limit [Å] | 3.691 | 3.691 |
| Number of reflections | 45955 | 7248 |
| <I/σ(I)> | 4.71 | 0.46 |
| Completeness [%] | 94.1 | |
| Redundancy | 1.84 | |
| CC(1/2) | 0.996 | 0.170 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 287 | 15 % PEG 3350 25% ethylene glycol |
