Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-07-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 135.469, 135.469, 35.579 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.382 - 1.700 |
| Rwork | 0.195 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ol0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.841 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.382 | 44.340 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
| Rmerge | 0.202 | 0.065 | 9.833 |
| Rmeas | 0.207 | 0.067 | 10.070 |
| Rpim | 0.045 | 0.015 | 2.166 |
| Number of reflections | 21730 | 192 | 1117 |
| <I/σ(I)> | 16 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 39.5 | 28.9 | 40.9 |
| CC(1/2) | 0.999 | 0.998 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.17 M ammonium sulfate, 25.5% PEG 4000, 15% glycerol |
