7QBT
B12-dependent radical SAM methyltransferase, Mmp10 with [4Fe-4S] cluster, cobalamin, and S-methyl-5'-thioadenosine bound.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 156.064, 163.915, 77.805 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.710 - 1.900 |
R-factor | 0.2026 |
Rwork | 0.203 |
R-free | 0.22910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7qbs |
RMSD bond length | 0.009 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.4 (16-JUL-2021)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.720 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.112 | |
Rpim | 0.031 | 0.847 |
Number of reflections | 137077 | 2742 |
<I/σ(I)> | 12.4 | 0.7 |
Completeness [%] | 99.8 | 97.2 |
Redundancy | 13.8 | 13.7 |
CC(1/2) | 0.999 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 20% (w/v) PEG8000, 100 mM Tris |