7QBS

B12-dependent radical SAM methyltransferase, Mmp10 with [4Fe-4S] cluster, cobalamin, S-adenosyl-L-methionine, and peptide bound.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857, 1.72200
Spacegroup name	P 63
Unit cell lengths	119.290, 119.290, 68.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.000 - 2.327
R-factor	0.1849
Rwork	0.182
R-free	0.23520
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	BUSTER (2.10.4 (16-JUL-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.390
High resolution limit [Å]	2.327	2.330
Rmerge	0.202
Rpim	0.045
Number of reflections	23924	479
<I/σ(I)>	11.6	0.8
Completeness [%]	99.6	95.2
Redundancy	20.7	18.2
CC(1/2)	0.999	0.283

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	23% (w/v) PEG8000, 100 mM Tris