7QBP

Crystal structure of R2-like ligand-binding oxidase from Saccharopolyspora Erythraea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 43 21 2
Unit cell lengths	103.173, 103.173, 61.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.050 - 1.380
R-factor	0.1522
Rwork	0.151
R-free	0.16540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ee4
RMSD bond length	0.018
RMSD bond angle	1.383
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.050	1.429
High resolution limit [Å]	1.380	1.380
Rmerge	0.063	1.782
Rmeas	0.064	1.818
Number of reflections	68755	6772
<I/σ(I)>	29.88	1.79
Completeness [%]	100.0	100
Redundancy	26.1
CC(1/2)	1.000	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	294	200 mM Lithium sulfate, 100 mM Sodium acetate pH 4.6, and 50 % (w/v) PEG 400