7QBK

Crystal structure of a second homolog of R2-like ligand-binding oxidase in Sulfolobus acidocaldarius (SaR2loxII)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.984
Spacegroup name	P 64
Unit cell lengths	128.029, 128.029, 41.894
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.910 - 2.260
R-factor	0.2034
Rwork	0.202
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hr0
RMSD bond length	0.003
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.910	2.341
High resolution limit [Å]	2.260	2.260
Rmerge	0.159	1.442
Rmeas	0.186	1.683
Rpim	0.095	0.850
Number of reflections	17196	1739
<I/σ(I)>	6.67	0.91
Completeness [%]	91.8	93.53
Redundancy	3.6	3.7
CC(1/2)	0.991	0.398

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	294	24% (w/v) polyethylene glycol 1500, 5% (v/v) formamide, 40 mM sodium propionate, 20 mM sodium cacodylate trihydrate, 40 mM bis-tris propane