7QBD

TC:CD320 in complex with nanobody TC-Nb26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	102.129, 102.129, 360.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.140 - 4.180
R-factor	0.2621
Rwork	0.262
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zrp
RMSD bond length	0.038
RMSD bond angle	2.004
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.240	4.330
High resolution limit [Å]	4.180	4.180
Number of reflections	28382	1406
<I/σ(I)>	10.17
Completeness [%]	95.0
Redundancy	2
CC(1/2)	0.999	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297.15	150 mM Ammonium citrate tribasic pH 7.0, 21% w/v PEG 3350