7Q9X
Crystal structure of Chromobacterium violaceum aminotransferase in complex with PLP-pyruvate adduct
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.330, 61.380, 116.480 |
| Unit cell angles | 103.56, 89.38, 105.42 |
Refinement procedure
| Resolution | 39.430 - 1.600 |
| Rwork | 0.227 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ba4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.422 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.430 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 156999 | 3529 |
| <I/σ(I)> | 14.9 | 2.5 |
| Completeness [%] | 76.1 | 23 |
| Redundancy | 4.7 | 4.3 |
| CC(1/2) | 0.999 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 290 | 12.5% PEG 8000 and 50 mM HEPES pH 7.5 10 mM PLP, 5 mM pyruvate |
