Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 55.788, 55.788, 114.842 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.360 - 2.500 |
| Rwork | 0.234 |
| R-free | 0.27590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tb5 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 1.016 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.870 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 7634 | 547 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.995 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 279 | 0.1 M citric acid pH 4.0 and 3.2 M AmSO4 |
