7Q6J

Crystal structure of the human GDAP1 CMT2 mutant-H123R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	72.710, 115.880, 116.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.380 - 2.200
R-factor	0.2162
Rwork	0.215
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7alm
Data reduction software	XDS (Feb 5, 2021)
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18-3861)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.380	45.380	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.072	0.020	1.602
Rmeas	0.079	0.022	1.738
Total number of observations	328536
Number of reflections	50431	640	3647
<I/σ(I)>	14.78	61.22	1.43
Completeness [%]	99.8	98.5	99.1
Redundancy	6.515	5.614	6.66
CC(1/2)	0.999	0.999	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 3350, DL-Malic Acid