7Q5Y
Structure of NADH:ubichinon oxidoreductase (complex I) of the hyperthermophilic eubacterium Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 132.880, 240.240, 230.920 |
| Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.700 |
| R-factor | 0.1714 |
| Rwork | 0.169 |
| R-free | 0.20870 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP (v2006/3) |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.128 | 1.689 |
| Number of reflections | 393067 | 20959 |
| <I/σ(I)> | 8.03 | |
| Completeness [%] | 98.1 | 98 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1 M sodium acetate (pH 5.0), 40% methylpentanediol (MPD), 0.05% decyl-beta-D-maltoside |
