7Q5T

The tandem SH2 domains of SYK with a bound FCER1G diphospho-ITAM peptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	144.136, 88.035, 158.486
Unit cell angles	90.00, 113.58, 90.00

Refinement procedure

Resolution	73.260 - 2.200
Rwork	0.207
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a81
RMSD bond length	0.008
RMSD bond angle	1.506
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.260	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.145	1.672
Rmeas	0.164	2.018
Rpim	0.077	1.100
Number of reflections	90843	3838
<I/σ(I)>	7.5	0.7
Completeness [%]	98.3	83.8
Redundancy	8.3	4.9
CC(1/2)	0.995	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	30 mM sodium nitrate, 30 mM dibasic sodium phosphate, 30 mM ammonium sulphate, 100 mM Tris/BICINE, 10% PEG 20,000, 20% PEG 500 MME