7Q5T
The tandem SH2 domains of SYK with a bound FCER1G diphospho-ITAM peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.136, 88.035, 158.486 |
| Unit cell angles | 90.00, 113.58, 90.00 |
Refinement procedure
| Resolution | 73.260 - 2.200 |
| Rwork | 0.207 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a81 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.506 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.260 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.145 | 1.672 |
| Rmeas | 0.164 | 2.018 |
| Rpim | 0.077 | 1.100 |
| Number of reflections | 90843 | 3838 |
| <I/σ(I)> | 7.5 | 0.7 |
| Completeness [%] | 98.3 | 83.8 |
| Redundancy | 8.3 | 4.9 |
| CC(1/2) | 0.995 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30 mM sodium nitrate, 30 mM dibasic sodium phosphate, 30 mM ammonium sulphate, 100 mM Tris/BICINE, 10% PEG 20,000, 20% PEG 500 MME |
