Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-31 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.343, 71.343, 200.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.304 - 2.717 |
Rwork | 0.274 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iwv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.858 |
Data reduction software | xia2 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.304 | 2.788 |
High resolution limit [Å] | 2.717 | 2.717 |
Rmeas | 0.025 | 0.830 |
Number of reflections | 14670 | 980 |
<I/σ(I)> | 16.9 | |
Completeness [%] | 99.7 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 294 | 30-60 mM Sodium Citrate, 50-70mM NaCl, 12-14 % (w/v) PEG-3350, 60-65 mM n-octyl-beta-d-glucoside, 6-12 mg/mL protein |