7Q4N
transcription factor CDX2 bound to hydroxymethylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97242 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 69.897, 46.634, 120.048 |
| Unit cell angles | 90.00, 98.25, 90.00 |
Refinement procedure
| Resolution | 43.450 - 3.200 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.27940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lty |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.264 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.450 | 43.410 | 3.210 |
| High resolution limit [Å] | 3.030 | 9.090 | 3.030 |
| Rmerge | 0.272 | 0.080 | 1.409 |
| Rmeas | 0.315 | 0.094 | 1.623 |
| Rpim | 0.155 | 0.048 | 0.788 |
| Total number of observations | 1036 | 4054 | |
| Number of reflections | 7406 | 298 | 1092 |
| <I/σ(I)> | 4.5 | 10.3 | 1.3 |
| Completeness [%] | 97.0 | 96.2 | 89.3 |
| Redundancy | 3.8 | 3.5 | 3.7 |
| CC(1/2) | 0.975 | 0.995 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 1500, potassium chloride, magnesium chloride, PEG 200, Bis-tris propane, 8.0 |
