7Q43

Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of dedicator of cytokinesis protein 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999997
Spacegroup name	P 43 21 2
Unit cell lengths	108.820, 108.820, 243.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.665 - 2.400
R-factor	0.205598267093
Rwork	0.202
R-free	0.26497
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.665	2.460
High resolution limit [Å]	2.400	2.400
Number of reflections	57945	4230
<I/σ(I)>	5.77
Completeness [%]	99.7
Redundancy	37.6713780309
CC(1/2)	0.983	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	hanging drop 20% PEG 3350 0.18M tris amonium citrate