7PYV

Crystal structure of human UBA6 in complex with the ubiquitin-like modifier FAT10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-09
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 1
Unit cell lengths	93.458, 93.593, 109.542
Unit cell angles	70.52, 88.54, 74.59

Refinement procedure

Resolution	45.140 - 3.270
R-factor	0.2166
Rwork	0.216
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pvn 6gf1 6gf2
RMSD bond length	0.005
RMSD bond angle	0.757
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.093	3.705
High resolution limit [Å]	3.268	3.268
Rmerge	0.202	1.092
Rmeas	0.236	1.266
Rpim	0.120	0.640
Number of reflections	30812	1542
<I/σ(I)>	5.2
Completeness [%]	59.1	53.4
Redundancy	3.84	3.9
CC(1/2)	0.988	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	277	0.5 M Lithium chloride, 0.1 M Tris pH 8.4, 25% PEG 6000