7PXT
Structure of an LPMO, collected from serial synchrotron crystallography data.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-15 |
| Detector | PSI JUNGFRAU 4M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 125.420, 125.420, 125.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.690 - 2.400 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.24760 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ach |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.690 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.293 | 0.910 |
| Number of reflections | 21952 | 1698 |
| <I/σ(I)> | 7.21 | 3.37 |
| Completeness [%] | 88.0 | 89.7 |
| Redundancy | 3.51 | 3.59 |
| CC(1/2) | 0.619 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 4.1 M NaCl, 0.1 M citric acid pH 4.0 The crystals were equilibrated in a drop of 3.5 M NaCl, 0.1 M citric acid pH 5.5 |
