7PV5

RBD domain of D. melanogaster tRNA (uracil-5-)-methyltransferase homolog A (TRMT2A)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-30
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0366445605250807
Spacegroup name	C 1 2 1
Unit cell lengths	92.410, 41.890, 48.820
Unit cell angles	90.00, 110.80, 90.00

Refinement procedure

Resolution	45.640 - 1.600
R-factor	0.1436
Rwork	0.141
R-free	0.19930
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.936
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.600	1.600
Number of reflections	23209	1684
<I/σ(I)>	11.63	3.51
Completeness [%]	99.7
Redundancy	6.5
CC(1/2)	0.998	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	2M AmSO4 and 2M NaCl