7PV1

Crystal structure of the dimeric mitofilin domain of Mic60 in complex with the CHCH domain of Mic19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	58.197, 85.371, 61.497
Unit cell angles	90.00, 101.72, 90.00

Refinement procedure

Resolution	40.738 - 2.497
R-factor	0.2334
Rwork	0.232
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pv0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.738	40.738	2.650
High resolution limit [Å]	2.500	7.420	2.500
Rmerge	0.112	0.024	1.655
Rmeas	0.130	0.028	1.911
Total number of observations	81921
Number of reflections	20339	804	3230
<I/σ(I)>	9.16	43.76	0.75
Completeness [%]	98.8	98.2	97.7
Redundancy	4.028	3.816	4.009
CC(1/2)	0.998	0.999	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% (w/v) Jeffamine ED-2001, 0.1 M HEPES/NaOH pH 7.0.