7PUR
mouse Interleukin-12 subunit beta - p80 homodimer in space group P21 crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.263, 158.334, 68.552 |
| Unit cell angles | 90.00, 100.21, 90.00 |
Refinement procedure
| Resolution | 39.620 - 3.900 |
| R-factor | 0.2852 |
| Rwork | 0.281 |
| R-free | 0.32370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sff |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.897 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.180 | 4.110 |
| High resolution limit [Å] | 3.880 | 3.880 |
| Rmeas | 0.325 | 1.900 |
| Number of reflections | 7714 | 1182 |
| <I/σ(I)> | 4.79 | 0.87 |
| Completeness [%] | 97.8 | 93 |
| Redundancy | 3.13 | 3.13 |
| CC(1/2) | 0.978 | 0.375 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287 | 5% MPD, 10% PEG6000, 100mM TRIS pH7.5 |
