7PUQ

CARM1 in complex with EML982

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	75.128, 98.694, 207.778
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.010 - 2.090
R-factor	0.1829
Rwork	0.181
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	2.120
High resolution limit [Å]	2.090	2.090
Rmerge	0.085	1.330
Rmeas	0.089	1.387
Rpim	0.024	0.386
Number of reflections	92014	4053
<I/σ(I)>	17.8	1.8
Completeness [%]	99.5	90.2
Redundancy	13.4	12
CC(1/2)	1.000	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	12% PEG 3350, 0.1 M NaCl, 0.1 M TrisHCl pH8.5