7PUL
Crystal structure of Endoglycosidase E GH20 domain from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.298, 57.046, 69.525 |
| Unit cell angles | 90.00, 92.97, 90.00 |
Refinement procedure
| Resolution | 44.077 - 1.400 |
| R-factor | 0.1591 |
| Rwork | 0.158 |
| R-free | 0.18290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rpm |
| RMSD bond length | 0.041 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.080 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.077 | 0.244 |
| Rmeas | 0.093 | 0.295 |
| Number of reflections | 60346 | 6021 |
| <I/σ(I)> | 11.92 | 2.12 |
| Completeness [%] | 99.7 | 99.52 |
| Redundancy | 3.2 | 3 |
| CC(1/2) | 0.991 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM bicine/Trizma base pH 8.5, 30 mM magnesium chloride hexahydrate, 30 mM calcium chloride dihydrate, 12.5% w/v PEG 1000, 12.5% PEG w/v 3350, 12.5% v/v MPD |
