7PUK
Crystal structure of Endoglycosidase E GH18 domain from Enterococcus faecalis in complex with Man5 product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 222.030, 54.550, 83.920 |
Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
Resolution | 45.730 - 2.690 |
R-factor | 0.2275 |
Rwork | 0.226 |
R-free | 0.26210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7puj |
RMSD bond length | 0.002 |
RMSD bond angle | 0.501 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.730 | 2.786 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.123 | 0.846 |
Rmeas | 0.141 | 0.972 |
Number of reflections | 26261 | 2438 |
<I/σ(I)> | 10.16 | 1.9 |
Completeness [%] | 98.8 | 92.8 |
Redundancy | 4.4 | 4.2 |
CC(1/2) | 0.992 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM Bis-Tris propane pH 6.5, 200 mM sodium nitrate, 20% w/v PEG 3350 |