7PUJ

Crystal structure of Endoglycosidase E GH18 domain from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9792
Spacegroup name	P 65
Unit cell lengths	112.150, 112.150, 64.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.820 - 1.752
R-factor	0.1879
Rwork	0.186
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mdv
RMSD bond length	0.024
RMSD bond angle	0.787
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.820	1.815
High resolution limit [Å]	1.752	1.752
Rmerge	0.060	0.908
Rmeas	0.062	0.946
Number of reflections	46562	4569
<I/σ(I)>	34.02	2.73
Completeness [%]	99.9	99.05
Redundancy	19.1	13
CC(1/2)	1.000	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20 mM zinc chloride, 20% w/v PEG 3350