7PUC

CARM1 in complex with EML981

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	75.322, 98.943, 208.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.130 - 2.190
R-factor	0.2037
Rwork	0.202
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.130	2.230
High resolution limit [Å]	2.190	2.190
Rmerge	0.158	2.821
Rmeas	0.164	2.950
Rpim	0.063	0.843
Number of reflections	80457	3971
<I/σ(I)>	11.1	1.1
Completeness [%]	99.2	87
Redundancy	13.3	11.7
CC(1/2)	0.999	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	15% PEG 3350, 200mM AMMONIUM SULFATE, 100mM TRIS HCL pH8.5