7PUC
CARM1 in complex with EML981
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.322, 98.943, 208.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.130 - 2.190 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih3 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 2.230 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.158 | 2.821 |
| Rmeas | 0.164 | 2.950 |
| Rpim | 0.063 | 0.843 |
| Number of reflections | 80457 | 3971 |
| <I/σ(I)> | 11.1 | 1.1 |
| Completeness [%] | 99.2 | 87 |
| Redundancy | 13.3 | 11.7 |
| CC(1/2) | 0.999 | 0.511 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% PEG 3350, 200mM AMMONIUM SULFATE, 100mM TRIS HCL pH8.5 |
